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Participation of endogenous IGF‐I and TGF‐β1 with enamel matrix derivative‐stimulated cell growth in human periodontal ligament cells
Author(s) -
Okubo K.,
Kobayashi M.,
Takiguchi T.,
Takada T.,
Ohazama A.,
Okamatsu Y.,
Hasegawa K.
Publication year - 2003
Publication title -
journal of periodontal research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.31
H-Index - 83
eISSN - 1600-0765
pISSN - 0022-3484
DOI - 10.1034/j.1600-0765.2003.01607.x
Subject(s) - enamel matrix derivative , alkaline phosphatase , osteocalcin , transforming growth factor beta , growth factor , cell growth , periodontal fiber , endocrinology , chemistry , transforming growth factor , medicine , osteoblast , endogeny , cell culture , microbiology and biotechnology , biology , biochemistry , regeneration (biology) , dentistry , in vitro , enzyme , receptor , genetics
Previous studies have provided the biological basis for the therapeutic use of enamel matrix derivative (EMD) at sites of periodontal regeneration. A purpose of this study is to determine effects of EMD on cell growth, osteoblastic differentiation and insulin‐like growth factor‐I (IGF‐I) and transforming growth factor‐β1 (TGF‐β1) production in human periodontal ligament cells (HPLC). We also examined participation of endogenous IGF‐I and TGF‐β1 with EMD‐stimulated cell growth in these cells. HPLCs used in this study were treated with EMD alone or in combination with antihuman IGF‐I antibody (anti‐hIGF‐I) or anti‐hTGF‐β1, recombinant human bone morphogenetic protein‐2 (rhBMP‐2), 1,25‐dihydroxyvitamin D 3 [1,25(OH) 2 D 3 ], rhTGF‐β1 or rhIGF‐I. After each treatment, cell growth, the production of IGF‐I and TGF‐β1 and the expression of osteoblastic phenotypes were evaluated. EMD stimulated cell growth in dose‐dependent and time‐dependent manners. EMD was also stimulated to express IGF‐I and TGF‐β1 at protein and mRNA levels. The EMD‐stimulated cell growth was partially suppressed by cotreatment with anti‐hIGF‐I or anti‐hTGF‐β1, and cell growth was also stimulated by treatment with rhIGF‐I or rhTGF‐β1. rhBMP‐2 stimulated alkaline phosphatase (ALPase) activity and ALPase mRNA expression, and 1,25(OH) 2 D 3 stimulated ALPase and osteocalcin mRNA expression. However, EMD showed no effect on the osteoblastic phenotypes expression. These results demonstrated that EMD has no appreciable effect on osteoblastic differentiation, however it stimulates cell growth and IGF‐I and TGF‐β1 production in HPLC, and that these endogenous growth factors partially relate to the EMD‐stimulated cell growth in HPLC.