Premium
Tumor necrosis factor‐alpha expression and detection of apoptosis at the site of chronic periodontitis in AIDS patients
Author(s) -
Vieira Beatriz Julião,
De Souza Andrezza Rodrigues,
Aarestrup Fernando Monteiro
Publication year - 2003
Publication title -
journal of periodontal research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.31
H-Index - 83
eISSN - 1600-0765
pISSN - 0022-3484
DOI - 10.1034/j.1600-0765.2003.00701.x
Subject(s) - tumor necrosis factor alpha , periodontitis , chronic periodontitis , apoptosis , medicine , alpha (finance) , necrosis , cancer research , immunology , pathology , biology , genetics , construct validity , nursing , patient satisfaction
Background: Apoptosis, or programmed cell death, is associated with the regulation of the life cell cycle of leukocytes in healthy and diseased states. Objectives: In the present study, we investigated the presence of apoptosis of mononuclear inflammatory cells in the periodontal lesion from adult periodontitis in healthy control patients and AIDS patients. Materials and methods: Tissue samples adjacent to a 5–6 mm gingival sulcus, measured with a periodontal probe, were obtained during routine periodontal surgical procedures. The direct immuno‐peroxidase of digoxigenin‐labeled genomic DNA method was used for in situ detection of apoptosis in gingival tissues. Results: Many tumor necrosis factor‐alpha (TNFα)‐positive cells, detected by immunohistochemistry method, were observed in gingival samples of both groups of patients. In addition, a significant lower number ( p < 0.05) of mononuclear apoptotic cells were observed in AIDS patients when compared with healthy control patients. Conclusion: These data suggested an important role of the apoptosis of mononuclear cells in the pathogenesis of chronic adult periodontitis in AIDS patients.