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Direct evidence of collagenolysis in chronic periodontitis
Author(s) -
Ma Jian,
Sorsa Timo,
Billinghurst Clark R.,
Poole Robin A.,
Kitti Uula,
Santavirta Seppo,
Konttinen Yrjö T.
Publication year - 2003
Publication title -
journal of periodontal research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.31
H-Index - 83
eISSN - 1600-0765
pISSN - 0022-3484
DOI - 10.1034/j.1600-0765.2003.00689.x
Subject(s) - periodontitis , chronic periodontitis , medicine , periodontal disease , dentistry
Background: There is no previous evidence that collagenases in chronic periodontitis excessively cleave collagen fibrils. Objectives: In this study the eventual presence of neoepitopes produced in such a cleavage were looked for. Methods: A polyclonal antibody, which recognizes collagenase‐cleaved collagen type I 3/4 carboxy‐terminal neoepitope (COL1‐3/4C), was used in avidin–biotin–peroxidase complex staining. Results: In addition, moderate staining was seen in connective tissue bordering to the sulcular and junctional epithelium, surrounding some of the fibroblasts and in some areas infiltrated by inflammatory mononuclear cells. COL1‐3/4C staining in chronic periodontitis was more extensive (6.3 ± 1.2%, n = 10) and intense than that observed in controls (1.6 ± 0.7%, n = 10, Unpaired Student's t ‐test, p < 0.01). Conclusions: It is concluded that collagenases produced by host cells contribute to periodontal tissue destruction and attachment loss.