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Expression of endothelin‐1 in gingival epithelial cells
Author(s) -
Yamamoto Eri,
Awano Shuji,
Koseki Takeyoshi,
Ansai Toshihiro,
Takehara Tadamichi
Publication year - 2003
Publication title -
journal of periodontal research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.31
H-Index - 83
eISSN - 1600-0765
pISSN - 0022-3484
DOI - 10.1034/j.1600-0765.2003.00668.x
Subject(s) - porphyromonas gingivalis , inflammation , immunohistochemistry , epithelium , messenger rna , endothelin 1 , reverse transcription polymerase chain reaction , microbiology and biotechnology , chemistry , periodontal pathogen , periodontitis , biology , pathology , immunology , medicine , gene , receptor , biochemistry
Objectives and background: Endothelin‐1 (ET‐1) is a 21‐amino acid peptide with multifunctional regulation. ET‐1 expresses in various cells during inflammation. The present study aimed to examine the ET‐1 expression in oral epithelial cells after infection with the periodontal pathogen and to investigate the presence of ET‐1 in human inflamed and uninflamed gingival tissues. Materials and methods: The KB cells were infected with Porphyromonas gingivalis and the expression level of ET‐1 was examined using reverse transcription‐polymerase chain reaction (RT‐PCR) and enzyme‐linked immunosorbent assay (ELISA). The immunohistochemical analysis of ET‐1 was performed in gingival tissues obtained from patients. In addition, the ET‐1 mRNA expression in each tissue was also investigated by RT‐PCR. Results: The expression of ET‐1 in KB cells was strongly induced by the P. gingivalis infection. On the other hand, the strong immunoreactivity for ET‐1 was observed in the epithelium and vascular endothelial cells of the inflamed gingival tissue. Furthermore, the level of ET‐1 mRNA was greater in the inflamed tissues. Conclusion: These results suggested that the expression level of ET‐1 in gingival epithelial cells might be enhanced during the periodontal inflammation.