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Hard tissue formation on dentin surfaces applied with recombinant human bone morphogenetic protein‐2 in the connective tissue of the palate
Author(s) -
Miyaji Hirofumi,
Sugaya Tsutomu,
Miyamoto Tetsuro,
Kato Katsuya,
Kato Hiroshi
Publication year - 2002
Publication title -
journal of periodontal research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.31
H-Index - 83
eISSN - 1600-0765
pISSN - 0022-3484
DOI - 10.1034/j.1600-0765.2002.01611.x
Subject(s) - connective tissue , dentin , dentistry , hard tissue , bone morphogenetic protein , chemistry , bone tissue , anatomy , medicine , pathology , biochemistry , gene
The purpose of this study was to evaluate whether hard tissue might be formed on dentin surfaces applied with recombinant human bone morphogenetic protein‐2 (rhBMP‐2) in palatal connective tissue. Fifty‐eight dentin blocks were prepared from rat roots, demineralized with 24% EDTA (pH 7.0), applied with 0, 50 and 100 μg/ml rhBMP‐2, and labeled as groups 0, 50 and 100. The dentin blocks were then transplanted into palatal connective tissue of rats, and specimens were prepared at two and four weeks after surgery for histologic and histomorphometric examinations. The results showed that the percentage of newly formed hard tissue in relation to the total dentin block surface length in groups 0, 50 and 100 was 0.0%, 2.8% and 4.4% at two weeks, and 0.0%, 1.6% and 12.8% at four weeks, respectively. New hard tissue formation in groups 50 and 100 was significantly promoted as compared to group 0 ( p < 0.01). These findings thus indicate that rhBMP‐2 application to dentin enhanced new hard tissue formation on dentin surfaces in the connective tissue of the palate.