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Cytokine regulation of syndecan‐1 and ‐2 gene expression in human periodontal fibroblasts and osteoblasts
Author(s) -
Worapamorn W.,
Tam S. P.,
Li H.,
Haase H. R.,
Bartold P. M.
Publication year - 2002
Publication title -
journal of periodontal research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.31
H-Index - 83
eISSN - 1600-0765
pISSN - 0022-3484
DOI - 10.1034/j.1600-0765.2002.01610.x
Subject(s) - syndecan 1 , platelet derived growth factor receptor , northern blot , cytokine , growth factor , beta (programming language) , cell culture , gene expression , transforming growth factor beta , biology , transforming growth factor , tgf beta 1 , periodontal fiber , tgf beta signaling pathway , endocrinology , medicine , microbiology and biotechnology , cell , immunology , gene , biochemistry , receptor , genetics , dentistry , computer science , programming language
Cell‐surface proteoglycans participate in several biological functions including interactions with a variety of growth factors and cytokines. Regulation of syndecan‐1 and ‐2 gene expression was investigated in human periodontal ligament fibroblasts (PDLF), osteoblasts (OB) and gingival fibroblasts (GF), in response to platelet‐derived growth factor (PDGF‐BB), transforming growth factor (TGF‐β 1 ), and interleukin (IL‐1β) by Northern blot analyses. We also compared the effect of PDGF‐BB and TGF‐β 1 , separately and in combination, in the prolonged presence of IL‐1β on the expression of both syndecan genes. The results demonstrated that the three cell lines regulated the expression of syndecan‐1 and ‐2 in response to growth factors and cytokines in different manners. These cell lines increased syndecan‐1 mRNA levels in response to either PDGF‐BB or TGF‐β 1 and decreased levels in response to IL‐1β. The effect of IL‐1β on syndecan‐1 mRNA synthesis was partially reversed after adding PDGF‐BB and TGF‐β 1 , separately or in combination, in the presence of IL‐1β. In contrast, syndecan‐2 mRNA level was markedly upregulated in response to either TGF‐β 1 or IL‐1β in OB when compared with the other two cell lines. However, the stimulatory effect of TGF‐β 1 on syndecan‐2 mRNA production in OB was abolished in the prolonged presence of IL‐1β. These findings lend support to the notion that syndecan‐1 and syndecan‐2 have distinct functions which correlate with their source and functions within the periodontium.