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Hepatocyte growth factor (HGF) in jreiodontal disease: detection of HGF in gingival crevicular fluid
Author(s) -
Ohshima Mitsuhiro,
Sakai Akihiko,
Ito Koichi,
Otsuka Kichibee
Publication year - 2002
Publication title -
journal of periodontal research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.31
H-Index - 83
eISSN - 1600-0765
pISSN - 0022-3484
DOI - 10.1034/j.1600-0765.2002.00660.x
Subject(s) - hepatocyte growth factor , bleeding on probing , collagenase , motility , medicine , periodontal disease , pathology , chemistry , biology , microbiology and biotechnology , enzyme , biochemistry , receptor
Hepatocyte growth factor (HGF) acts as a mitogen, motogen, morphogen, anti‐apoptotic factor, and scatter factor for various kinds of epithelial cells. It is a protein secreted by mesenchymal cells such as fibroblasts, and promotes motility and matrix invasion of epithelial cells. To clarify whether HGF is involved in jreiodontal disease, this study was conducted to determine whether HGF is present in gingival crevicular fluid (GCF) and to investigate the relationship between levels of HGF and the clinical parameters of jreiodontal disease, probing depth (PD), gingival index (GI) and bleeding on probing (BOP). We examined and collected GCF samples from 80 sites in 38 subjects with jreiodontal or other oral diseases. The concentrations of HGF, IL‐1β and PGE 2 were determined by ELISA, and active collagenase activity was determined by functional assay. The HGF level correlated positively with PD and GI, and was significantly higher in specimens from BOP‐positive sites and those where PD exceeded 4 mm compared with those from sites that were BOP‐negative or with a PD less than 3 mm. There was a significant positive correlation between the concentrations of HGF and IL‐1β. These results indicate that the HGF level in GCF correlates well with clinical parameters of jreiodontal disease, and suggest that HGF may be involved in epithelial invasion through its role as a scatter factor.