Premium
Individual diversities in interferon gamma production by human peripheral blood mononuclear cells stimulated with periodontopathic bacteria
Author(s) -
Kobayashi Hiroaki,
Nagasawa Toshiyuki,
Aramaki Maya,
Mahada Rangsini,
Ishikawa Isao
Publication year - 2000
Publication title -
journal of periodontal research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.31
H-Index - 83
eISSN - 1600-0765
pISSN - 0022-3484
DOI - 10.1034/j.1600-0765.2000.035006319.x
Subject(s) - peripheral blood mononuclear cell , actinobacillus , porphyromonas gingivalis , immune system , immunology , microbiology and biotechnology , antibody , interferon gamma , lipopolysaccharide , biology , cytokine , periodontitis , medicine , in vitro , biochemistry
Polarization of type 1 (Th1) or type 2 (Th2) immune responses determines the prognosis of many infectious diseases. Interferon gamma (IFN‐γ) and IL‐4 are key cytokines for the development of type 1 and type 2 immune responses, respectively. The aim of this study was to examine individual diversities in the polarization of type 1 and type 2 responses against periodontopathic bacteria. Peripheral blood mononuclear cells (PBMCs) from adult periodontitis (AP) patients and healthy (H) subjects were stimulated with Porphyromonas gingivalis , Actinobacillus actinomycetemcomitans and Bacteroides forsythus with or without polymyxin‐B, CTLA‐4 Ig and anti‐IL‐12 antibody. IFN‐γ, IL‐4 and IL‐12 in the culture supernatant were measured. IFN‐γ and IL‐4 producing cells were also examined using a multiparameter flow cytometric assay. Bone resorption rate in AP patients was calculated using Schei's method, and the probing pocket depth was also measured. PBMCs from AP patients and H subjects produced IFN‐γ and IL‐12, whereas the production of IL‐4 was rarely observed. Among the bacteria tested, A. actinomycetemcomitans was the most potent inducer of IFN‐γ and IL‐12, and the reaction was inhibited by polymyxin‐B. IFN‐γ was found to be produced by T cells in the PBMCs, and the production was significantly reduced by CTLA‐4 Ig and anti‐IL‐12 neutralizing antibody. The amount of IFN‐γ produced by the PBMCs of AP patients and H subjects varied among individuals, and was significantly correlated with the amount of IL‐12 produced in a particular individual. The production of IFN‐γ was not related with periodontal condition which was evaluated using bone resorption and pocket depth. These results suggest that polarization of type 1 response against periodontopathic bacteria is dependent on the production of IL‐12 by monocytes, and that IL‐12 stimulates IFN‐γ production. However, individual diversities of IFN‐γ production might not be directly related to the severity of periodontitis.