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The fibrous structure of the cemento–dentinal junction in human molars shown by scanning electron microscopy combined with NaOH‐maceration
Author(s) -
Yamamoto T.,
Domon T.,
Takahashi S.,
Islam M. N.,
Suzuki R.
Publication year - 2000
Publication title -
journal of periodontal research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.31
H-Index - 83
eISSN - 1600-0765
pISSN - 0022-3484
DOI - 10.1034/j.1600-0765.2000.035002059.x
Subject(s) - maceration (sewage) , fibril , dentinal tubule , cementum , scanning electron microscope , molar , materials science , chemistry , collagen fibril , dentin , anatomy , composite material , dentistry , medicine , biochemistry
The cemento–dentinal junction was studied in acellular and cellular cementum of human mandibular third molars by scanning electron microscopy combined with NaOH‐maceration. Scanning electron microscopy with NaOH‐maceration was applied to observe the fibrous structure in detail through long sections of the cemento–dentinal junction. In macerated specimens, the cemento–dentinal junction was a fibril‐poor groove. Some cemental fibrils or fibril bundles penetrated the groove and appeared to intermingle with dentinal fibrils. Prolonged maceration caused detachment of the cemento–dentinal junction irrespective of fibril intermingling allowing observation of the inner cementum surface facing the root dentin. Observations suggested that the fibril intermingling was point‐like and present only in places at the cemento–dentinal junction. It was established that NaOH‐maceration removes interfibrillar substances effectively in connective tissues and does no damage to the collagen fibril structure and architecture. This study showed the 3‐dimensional fibrous structure of the cemento–dentinal junction in human mandibular third molars, and suggested that interfibrillar adhesive substances are more important than the fibril intermingling for the cemento–dentinal attachment.