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Morphology of azurophil lysosomes in polymorphonuclear leukocytes from humans with rapidly progressive periodontitis
Author(s) -
Pippin David J.,
Swafford James R.,
McCunniff Michael D.
Publication year - 2000
Publication title -
journal of periodontal research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.31
H-Index - 83
eISSN - 1600-0765
pISSN - 0022-3484
DOI - 10.1034/j.1600-0765.2000.035001026.x
Subject(s) - azurophilic granule , lysosome , granulocyte , periodontitis , intracellular , biology , immunology , pathology , peripheral blood , microbiology and biotechnology , chemistry , medicine , myeloperoxidase , biochemistry , enzyme , inflammation
Previous investigations have shown that subjects with rapidly progressive periodontitis (RPP), an early‐onset aggressive form of periodontitis, have polymorphonuclear leukocytes (PMNs) with increased intracellular levels of β‐glucuronidase, a characteristic enzyme of azurophil lysosomes. The current study attempted to account for that increase. Ten healthy controls and 10 otherwise healthy subjects with RPP participated. PMNs from peripheral blood were separated, fixed and reacted for peroxidase to identify azurophil lysosomes. Using transmission electron microscopy, 20 PMNs per subject were photographed at 10 000×. Photographs were subsequently digitized and analyzed by computer. RPP PMNs had a higher percentage of the area of the cell profile occupied by azurophil lysosomes compared to control subjects’ PMNs. The RPP subjects also had greater absolute numbers of azurophil lysosomes per cell. Lysosome shape was assessed visually. There were no differences between RPP and control groups for lysosome shape, with the majority of lysosomes in each group exhibiting a round or oval shape. RPP lysosomes did exhibit a significantly greater mean size.M