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Amine fluoride gel affects the viability and the generation of superoxide anions in human polymorphonuclear leukocytes: an in vitro study
Author(s) -
KnollKöhler Elisabeth,
Stiebel Juliane
Publication year - 2002
Publication title -
european journal of oral sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.802
H-Index - 93
eISSN - 1600-0722
pISSN - 0909-8836
DOI - 10.1034/j.1600-0722.2002.21259.x
Subject(s) - trypan blue , superoxide , chemistry , staurosporine , egta , biochemistry , in vitro , protein kinase c , nuclear chemistry , microbiology and biotechnology , calcium , kinase , enzyme , biology , organic chemistry
Amine hydrofluorides are widely used to prevent caries. As an acidulated gel, they were also studied for their applicability to reduce pathogenic bacteria in periodontal pockets. We assessed the toxicity of this pharmaceutical amine hydrofluoride preparation on human polymorphonuclear leukocytes in vitro by measuring Trypan blue exclusion and the generation of superoxide anions (O 2 – ) by the chemotactic peptide N ‐formyl‐ l ‐methionyl‐ l ‐leucyl‐ l ‐phenylalanine (fMLP) after a 3‐min contact with gel. Depending on the experimental conditions, gel dilutions up to 1.3 × 10 4 resulted in an increase in Trypan blue‐colored cells and liberation of β‐glucuronidase. Dilutions between 3 × 10 4 and 1 × 10 5 augmented the fMLP‐mediated O 2 – generation, which could be prevented by Ca 2+ chelation with BAPTA‐AM (1,2′‐bis ( o ‐aminophenoxy‐ethane‐ n , n , n ′, n ′‐tetraacetic acid tetra (acetoxymethyl) ester) and ethyleneglycoltetraacetic acid (EGTA) or inhibition of protein kinase C (PKC) with staurosporine and bisindolylmaleimide I, respectively. Compared with data published on the minimal inhibitory concentration for periodontal pathogenic bacteria, the cytotoxicity of amine hydrofluorides on eukaryotic cells is much greater and thus of consequence for their clinical use.