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Separation of the outer membrane and identification of major outer membrane proteins from Porphyromonas gingivalis
Author(s) -
Murakami Yukitaka,
Imai Masashi,
Nakamura Hiroshi,
Yoshimura Fuminobu
Publication year - 2002
Publication title -
european journal of oral sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.802
H-Index - 93
eISSN - 1600-0722
pISSN - 0909-8836
DOI - 10.1034/j.1600-0722.2002.11171.x
Subject(s) - bacterial outer membrane , porphyromonas gingivalis , membrane protein , gel electrophoresis , biology , sodium dodecyl sulfate , polyacrylamide gel electrophoresis , differential centrifugation , escherichia coli , microbiology and biotechnology , membrane , chemistry , biochemistry , bacteria , enzyme , genetics , gene
The outer membrane of Porphyromonas gingivalis , an oral strict anaerobe, was isolated by sucrose density gradient centrifugation. The outer membrane obtained by the differential detergent extraction method, previously reported, showed an essentially similar protein pattern on sodium dodecyl sulfate‐polyacrylamide gel electrophoresis (SDS‐PAGE), confirming that the latter method is suitable for the study of outer membrane proteins in this organism. N‐terminal amino acid sequence analysis revealed that major outer membrane proteins in this organism included Arg‐gingipain, Lys‐gingipain, RagA (a TonB‐linked receptor), and putative porins that were homologous to Escherichia coli OmpA.