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Actinobacillus actinomycetemcomitans ‐induced expression of IL‐1α and IL‐1β in human gingival epithelial cells: role in IL‐8 expression
Author(s) -
Sfakianakis Andreas,
Barr Charles E.,
Kreutzer Don L.
Publication year - 2001
Publication title -
european journal of oral sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.802
H-Index - 93
eISSN - 1600-0722
pISSN - 0909-8836
DOI - 10.1034/j.1600-0722.2001.00162.x
Subject(s) - actinobacillus , in vitro , interleukin , periodontium , epithelium , in vivo , secretion , interleukin 8 , immunohistochemistry , chemistry , microbiology and biotechnology , immunology , inflammation , biology , cytokine , periodontitis , medicine , pathology , dentistry , biochemistry
Gingival epithelial cells (GEC) are the first cells of the host that encounter the periodontal pathogens, and therefore their role in the initiation of the inflammatory response is critical. We aimed to: 1) characterize the expression of interleukin (IL)‐1α and IL‐1β in human gingiva and cultured GEC; 2) demonstrate the ability of A. actinomycetemcomitans extracts to upregulate IL‐1α, IL‐1β and IL‐8 expression in GEC in vitro; and 3) characterize the role of IL‐1α and IL‐1β in the induction of IL‐8 expression in GEC in vitro . Ten gingival biopsies (5 inflamed and 5 controls) and cultured GEC were examined for IL‐1α and IL‐1β using immunohistochemical techniques. GEC were also challenged with A. actinomycetemcomitans extracts or IL‐1, and secretion of IL‐1 and IL‐8 was determined by ELISA. In vivo , IL‐1α and IL‐1β were localized in the gingival epithelium and the infiltrating leukocytes. In vitro, A. actinomycetemcomitans extracts induced a time‐dependent expression of IL‐1α, IL‐1β and IL‐8 in GEC. IL‐1 inhibitors did not affect A. actinomycetemcomitans ‐induced IL‐8, although they inhibited IL‐8 induced by IL‐1α or IL‐1β. In conclusion, GEC are a major source of IL‐1α and IL‐1β in the periodontium, which in turn induce additional inflammatory mediators such as IL‐8. Therefore GEC can be a potential target for therapeutic intervention in the future.