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Bactericidal effect of delmopinol on attached and planktonic Streptococcus sanguinis cells
Author(s) -
Burgemeister Stefan,
Decker EvaMaria,
Weiger Roland,
Brecx Michel
Publication year - 2001
Publication title -
european journal of oral sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.802
H-Index - 93
eISSN - 1600-0722
pISSN - 0909-8836
DOI - 10.1034/j.1600-0722.2001.00121.x
Subject(s) - streptococcus sanguinis , bacteria , biofilm , chemistry , streptococcus mutans , microorganism , microbiology and biotechnology , fluorescent staining , in vitro , streptococcus , staining , biology , biochemistry , genetics
The aim of this investigation was to determine the antibacterial effect of varying concentrations of delmopinol‐HCl on attached as well as on planktonic Streptococcus sanguinis cells in vitro . In addition, a possible antiadhesive effect on attached micro‐organisms was to be investigated. S. sanguinis cells were allowed to attach to glass surfaces. These as well as planktonic cells were exposed to delmopinol‐HCl in concentrations ranging from 0.2% to 0.00005% for 2 min. The percentage of vital bacteria was calculated by means of a fluorescence staining method. Total counts of attached bacteria were performed to determine any possible detaching effect by the delmopinol‐HCl. The CFU were determined for the planktonic bacteria. Attached as well as planktonic bacteria showed a marked decrease in vitality following exposure to 0.2% delmopinol‐HCl. After exposure to 0.05% this was only the case with the attached microorganisms. The total number of attached bacteria was not reduced by the delmopinol treatment. During initial dental biofilm formation, delmopinol‐HCl causes a bactericidal effect when applied in concentrations of 0.05% and higher.