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Effect of fluoride on glucose incorporation and metabolism in biofilm cells of Streptococcus mutans
Author(s) -
Balzar Ekenbäck Susann,
Linder Lars E.,
Sund MarieLouise,
Lönnies Helena
Publication year - 2001
Publication title -
european journal of oral sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.802
H-Index - 93
eISSN - 1600-0722
pISSN - 0909-8836
DOI - 10.1034/j.1600-0722.2001.00005.x
Subject(s) - streptococcus mutans , fluoride , biofilm , sodium fluoride , chemistry , lactic acid , saliva , microbiology and biotechnology , metabolism , fluoride varnish , varnish , bacteria , nuclear chemistry , biochemistry , inorganic chemistry , biology , organic chemistry , genetics , coating
The aim of this study was two‐fold: firstly, to study the effect of high fluoride concentrations on carbohydrate metabolism in Streptococcus mutans present in biofilms on hydroxyapatite; and, secondly, to evaluate the effect of fluoride‐bound hydroxyapatite on lactic acid formation in growing biofilms of Strep. mutans . Biofilms of a clinical strain of Strep. mutans on saliva‐coated hydroxyapatite beads were incubated with sodium fluoride over a wide range of concentrations. At high fluoride concentrations (>10 mM) the incorporation of [ 14 C]‐labeled glucose decreased by 80–85%, at both pH 7.0 and 5.6. At lower fluoride concentrations, the effect of fluoride on the incorporation of labeled glucose was pH‐dependent in both biofilm cells and in planktonic cells. At pH 7.0, fluoride at concentrations <10 mM had little or no effect. Pretreatment of hydroxyapatite discs with fluoride varnish (Fluor Protector) or fluoride solutions caused a statistically significant reduction of lactic acid formation in associated, growing biofilms of Strep. mutans . Fluoride varnish and 0.2% (47.6 mM) sodium fluoride solution exhibited a statistically significant inhibitory effect on lactate production.