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Quantitative image analysis of hyaluronan expression in human tooth germs
Author(s) -
Felszeghy Szabolcs,
Hyttinen Mika,
Tammi Raija,
Tammi Markku,
Módis László
Publication year - 2000
Publication title -
european journal of oral sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.802
H-Index - 93
eISSN - 1600-0722
pISSN - 0909-8836
DOI - 10.1034/j.1600-0722.2000.108004311.x
Subject(s) - ameloblast , odontoblast , dental papilla , dentin , enamel paint , pulp (tooth) , amelogenesis , amelogenin , matrix (chemical analysis) , chemistry , basement membrane , microbiology and biotechnology , anatomy , dentistry , biology , medicine , chromatography
The expression of hyaluronan in human tooth germs was studied by using a biotinylated hyaluronan‐binding complex and quantitative digital image analysis. At the cap stage, dental papilla exhibited a moderate staining, while intense reaction was observed in the apical portion of presecretory ameloblasts, stellate reticulum, and in dental basement membrane. When the enamel and dentine matrices started to develop, a strong hyaluronan reaction was evident in the young enamel and the apical portion of secretory ameloblasts. No hyaluronan could be detected in the secretory ameloblasts and enamel matrix of the early (9‐wk‐old) post‐natal stage. It is concluded that hyaluronan may play a transitory role in the early phase of the development of the enamel matrix organization. A very weak signal was observed in the wall of dentin tubules, whereas the rest of the dentine matrix was not stained. The odontoblasts and the pulp were also moderately stained, and these reactions gradually decreased with age, suggesting that hyaluronan may also contribute to the development of dentine matrix and pulp.