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Treatment of inflamed ferret dental pulps with recombinant bone morphogenetic protein‐7
Author(s) -
Rutherford R. Bruce,
Gu Keni
Publication year - 2000
Publication title -
european journal of oral sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.802
H-Index - 93
eISSN - 1600-0722
pISSN - 0909-8836
DOI - 10.1034/j.1600-0722.2000.108003202.x
Subject(s) - recombinant dna , bone morphogenetic protein , pulpitis , dentinogenesis , bone morphogenetic protein 7 , pulp (tooth) , dentistry , medicine , chemistry , odontoblast , pathology , biochemistry , gene
Recombinant human BMP‐7 (bone morphogenetic protein‐7, osteogenic protein‐1) is osteogenic, dentinogenic and cementogenic when implanted into the appropriate tissue in vivo . However, most studies characterizing the induction of these tissues have implanted BMP‐7 into freshly surgerized, clinically healthy tissues. To determine if BMP‐7 is dentinogenic in inflamed dental pulps, we applied BMP‐7 to inflamed ferret pulps. A single application of 5 μ g of a commercial preparation of lipopolysaccharide (LPS) from Salmonella typhimurium directly to the coronal pulp induced a reversible mixed inflammatory exudate of moderate intensity within 3 d. Treatment with a single application of 2.5, 7.5 or 25 μ g recombinant human BMP‐7/mg collagen (2 mg total mass/tooth) induced reparative dentinogenesis in controls but not LPS treated dental pulps. These data reveal that a single application of up to 50 μ g/tooth of exogenous recombinant BMP‐7 is insufficient to induce reparative dentinogenesis in ferret teeth with reversible pulpitis. Given that pulp cells in the inflamed tissues likely retain the capacity to respond to exogenous BMP‐7, it is possible that insufficient active recombinant protein is available to induce tissue formation in experimentally inflamed dental pulps.