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Loss of heterozygosity at APC and MCC genes of oral cancer and leukoplakia tissues from Indian tobacco chewers
Author(s) -
Sikdar Nilabja,
Paul Ranjan Rashmi,
Panda Chinmay K.,
Banerjee Sanat K.,
Roy Bidyut
Publication year - 2003
Publication title -
journal of oral pathology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.887
H-Index - 83
eISSN - 1600-0714
pISSN - 0904-2512
DOI - 10.1034/j.1600-0714.2003.00132.x
Subject(s) - loss of heterozygosity , leukoplakia , carcinogenesis , cancer , medicine , adenomatous polyposis coli , tumor suppressor gene , pathology , colorectal cancer , cancer research , gene , biology , genetics , allele
Background: Loss of heterozygosity (LOH) at tumor suppressor genes, such as adenomatous polyposis coli (APC) and mutated in colon cancer (MCC) genes, is one of the early events in carcinogenesis of oral tissue in Caucasian and Chinese patients. We wanted to check whether it is also true in Indian oral pre‐cancer and cancer patients. Methods: Loss of heterozygosity at APC and MCC genes was investigated in 57 and 40 unrelated primary oral leukoplakia (a pre‐cancerous lesion) and squamous cell carcinomas (SCC), respectively, by polymerase chain reaction. Results: In these samples, most of the leukoplakia patients had tobacco smoking habit whereas majority of cancer patients had tobacco chewing habit. LOH at APC gene was observed in 4 of 16 (25%) and 1 of 29 (3%) informative tumor and leukoplakia DNAs from tobacco chewers, respectively. LOH at MCC gene was not detected either in tumor or in leukoplakia DNAs. Conclusion: This infrequent LOH at APC gene of pre‐cancer and cancer tissues suggests that it may not be an early event in oral carcinogenesis in these patients.