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Immunohistochemical detection of matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs) in ameloblastomas
Author(s) -
Kumamoto Hiroyuki,
Yamauchi Kensuke,
Yoshida Mitsuhide,
Ooya Kiyoshi
Publication year - 2003
Publication title -
journal of oral pathology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.887
H-Index - 83
eISSN - 1600-0714
pISSN - 0904-2512
DOI - 10.1034/j.1600-0714.2003.00086.x
Subject(s) - matrix metalloproteinase , basement membrane , pathology , mesenchymal stem cell , immunohistochemistry , stromal cell , ameloblastoma , biology , medicine , molar , dentistry , biochemistry
Background: To evaluate the roles of matrix metalloproteinases (MMPs) and their tissue inhibitors (TIMPs) in tumor progression, expression of MMP‐1, ‐2 and ‐9 and TIMP‐1 and ‐2 was analyzed in ameloblastomas as well as tooth germs. Methods: Frozen tissue sections of seven tooth germs and 22 ameloblastomas were immunohistochemically examined using anti‐MMP‐1, ‐2 and ‐9 and anti‐TIMP‐1 and ‐2 antibodies. Results: MMP‐1, ‐2 and ‐9 and TIMP‐1 and ‐2 were expressed strongly in mesenchymal components of tooth germs, and stromal cells of ameloblastomas. Immunoreactivity for MMP‐9 in stromal cells of ameloblastomas was significantly stronger than in mesenchymal cells of dental follicles and dental papillae. Dental laminae showed weak MMP‐2 expression in six tooth germs, MMP‐9 expression in two tooth germs and TIMP‐1 expression in six tooth germs. Some tumor cells showed weak MMP‐2 expression in 19 ameloblastomas, MMP‐9 expression in four ameloblastomas and TIMP‐1 expression in all cases. TIMP‐2 reactivity was prominently found in basement membrane zones of dental laminae in tooth germs, and tumor cell islands or nests in ameloblastomas. Conclusion: Expression of MMPs and TIMPs was considered to be associated with interactions between epithelial cells and mesenchymal components in normal and neoplastic odontogenic tissues; these molecules might play a role in regulation of tumor progression in ameloblastomas as well as regulation of developmental processes in tooth germs.