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Usefulness of AgNOR counts in diagnosing epithelial dysplasia
Author(s) -
Ray Jay G.,
Chattopadhyay Amit,
Caplan Daniel J.
Publication year - 2003
Publication title -
journal of oral pathology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.887
H-Index - 83
eISSN - 1600-0714
pISSN - 0904-2512
DOI - 10.1034/j.1600-0714.2003.00053.x
Subject(s) - dysplasia , medicine , gold standard (test) , h&e stain , pathology , epithelial dysplasia , stain , nucleolus organizer region , staining , nucleus , psychiatry , nucleolus
Background: Diagnosis of epithelial dysplasia has traditionally been subjective, and there is a need for a quantifiable and useful test. Methods: In a double blind study, clinical leukoplakias from 52 people were diagnosed for presence (DLK) or absence (NDLK) of epithelial dysplasia using hematoxylin and eosin (H&E) stain as a gold standard criterion, and results were compared against their mean silver stainable nucleolar organizer region (AgNOR) counts. We used mean AgNOR count cut‐point of 2.37 from our prior report as the diagnostic threshold (mean ≥ 2.37 being DLK and mean < 2.37 being NDLK). Results: The two methods (H&E and AgNOR) disagreed in 37% of the diagnoses. Both NDLK and DLK had high AgNOR counts. P‐AgNOR was non‐contributory for diagnosing epithelial dysplasia. Conclusions: Mean AgNOR count can be a useful tool in definitive diagnosis of epithelial dysplasia.