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Interleukin‐1β induces interleukin‐6 mRNA expression and protein production in synovial cells from human temporomandibular joint
Author(s) -
Ogura Naomi,
Tobe Makiko,
Sakamaki Hiroyuki,
Kujiraoka Hiroaki,
Akiba Masakazu,
Abiko Yoshimitsu,
Nagura Hideaki
Publication year - 2002
Publication title -
journal of oral pathology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.887
H-Index - 83
eISSN - 1600-0714
pISSN - 0904-2512
DOI - 10.1034/j.1600-0714.2002.310606.x
Subject(s) - temporomandibular joint , synovial membrane , immunocytochemistry , interleukin , messenger rna , microbiology and biotechnology , cell culture , chemistry , pathology , biology , immunology , cytokine , medicine , inflammation , biochemistry , genetics , gene
Abstract Background: Interleukin (IL)‐1β and IL‐6 were found to be elevated in fluid from the temporomandibular joint (TMJ), although the source of these cytokines was not elucidated. There is little known about the function and response of synovial cells in the TMJ. The purpose of this study was to prepare cultured human synovial cells (HTS cells) from the TMJ and to investigate IL‐6 production in HTS cells incubated with IL‐1β. Methods: HTS cells were isolated from temporomandibular joint synovial tissue using an outgrowth method and then cultured in Ham's F12 medium containing 10% fetal bovine serum. The HTS cells were treated with or without IL‐1β for 3, 6, 9 and 24 h. IL‐6 and soluble IL‐6 receptor (sIL‐6R) levels in cultured supernatant were measured by ELISA. IL‐6 mRNA expression was investigated using immunocytochemistry and RT‐PCR. Results: HTS cells were morphologically heterogeneous. IL‐1β increased IL‐6 production in HTS cells. In those treated with IL‐1β, several cells were strongly stained in the cytoplasm around the nucleus, while several cells were weakly stained in this area. IL‐1β also stimulated IL‐6 mRNA expression. In contrast, sIL‐6R could not be detected in cells treated with or without IL‐1β. Conclusions: IL‐1β increased IL‐6 production in synovial cells resulting from an increase in IL‐6 mRNA expression. Enhanced production of IL‐6, which is associated with bone resorption and inflammatory response, seems to be related to the progression of TMJ disorders.