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Involvement of substance P, mast cells, TNF‐α and ICAM‐1 in the infiltration of inflammatory cells in human periapical granulomas
Author(s) -
Kabashima Hiroaki,
Nagata Kengo,
Maeda Katsumasa,
Iijima Tadahiko
Publication year - 2002
Publication title -
journal of oral pathology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.887
H-Index - 83
eISSN - 1600-0714
pISSN - 0904-2512
DOI - 10.1034/j.1600-0714.2002.310309.x
Subject(s) - pathology , inflammation , tumor necrosis factor alpha , periodontitis , substance p , proinflammatory cytokine , medicine , pathogenesis , immunohistochemistry , infiltration (hvac) , immunology , receptor , physics , neuropeptide , thermodynamics
Background: Substance P (SP), a potent proinflammatory peptide present in sensory neurons, is believed to be a major mediator of neurogenic inflammation. The aim of this study was to examine the localization and involvement of SP, mast cells and tumor necrosis factor‐α (TNF‐α)‐positive cells in human periapical granulomas. Methods: Sections from seven periapical granulomas were stained using a variety of immunohistochemical methods. Results: SP‐immunoreactive nerve fibers, mast cells and TNF‐α‐positive cells were found localized in the vicinity of blood vessels in all the samples of periapical granulomas. The vascular endothelial cells stained positively for E‐selectin and intercellular adhesion molecule‐1. SP, TNF‐α‐positive cells and E‐selectin could not be detected in clinically healthy periodontal ligament, and served as a negative control. Conclusion: Our findings suggest that SP, mast cells, TNF‐α‐positive cells and E‐selectin may modulate the pathogenesis of apical periodontitis and may be responsible for stimulating the formation of granuloma with the resorption of periapical bone.