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Distribution of basement membrane type IV collagen α chains in ameloblastoma: an immunofluorescence study
Author(s) -
Nakano Keisuke,
Siar Chong Huat,
Nagai Noriyuki,
Naito Ichiro,
Sado Yoshikazu,
Nagatsuka Hitoshi,
Hoh Choufuku,
Kurada Katsuhiro,
Tsujigiwa Hidetsugu,
Gunduz Mehmet
Publication year - 2002
Publication title -
journal of oral pathology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.887
H-Index - 83
eISSN - 1600-0714
pISSN - 0904-2512
DOI - 10.1034/j.1600-0714.2002.00162.x
Subject(s) - basement membrane , ameloblastoma , epithelium , pathology , type iv collagen , adamantinoma , chemistry , immunofluorescence , biology , odontogenic tumor , microbiology and biotechnology , immunology , anatomy , antibody , medicine , extracellular matrix , lesion , laminin , maxilla
Background: Type IV collagen, a heterotrimeric molecule that exists in six genetically distinct forms, α1(IV)–α6(IV) is a major structural component of basement membrane (BM) and acts as a scaffold for other BM constituents. Methods: Indirect immunofluorescence using α chain‐specific monoclonal antibodies was employed to clarify basement membrane (BM) collagen IV distribution in two ameloblastoma, and for comparison, on oral mucosa and tooth germ. Results: Ameloblastoma BM expressed five of six genetically distinct forms of collagen IV: α1(IV), α2(IV), α5(IV) and α6(IV) chains occurred as intense linear stainings without disruption around neoplastic epithelium, and this expression pattern was fundamentally similar to oral mucosa BM; α4(IV) expression was rare and occurred around nests of primitive tumor cells or potentially invasive sites. The tooth germ demonstrated a stage‐ and position‐specific collagen IV distribution: the inner enamel epithelium BM expressed α1(IV), α2(IV), and α4(IV) except in the cuspal predentine region; and the outer enamel epithelium BM expressed α1(IV), α2(IV), α5(IV), and α6(IV) chains. Conclusions: Results suggest that collagen IV α chain distribution in ameloblastoma BM plays an important role in tumor cytodifferentiation and progression.