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Analysis of the Ki‐67 antigen at the invasive tumour front of human oral squamous cell carcinoma
Author(s) -
Tumuluri V.,
Thomas G. A.,
Fraser I. S.
Publication year - 2002
Publication title -
journal of oral pathology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.887
H-Index - 83
eISSN - 1600-0714
pISSN - 0904-2512
DOI - 10.1034/j.1600-0714.2002.00042.x
Subject(s) - ki 67 , pathology , antigen , grading (engineering) , epithelium , staining , basal cell , cell , medicine , immunohistochemistry , biology , immunology , ecology , genetics
Background: It is hypothesised that cell proliferation, as measured by the Ki‐67 labelling index (LI) at the invasive tumour front (ITF) was directly related to the histological grade in human oral squamous cell carcinomas (SCCs). Methods: Tissues from 42 human oral SCCs were collected and stained with an antibody directed against the Ki‐67 antigen using an advanced polymer staining system. Quantitation of the immunopositive cells was performed on two parallel sections at the invasive tumour front (ITF), using an image analyser. The Ki‐67 LI was expressed as the number of positive nuclei/mm 2 of epithelium. The control tissue used was normal epithelium at the excision margin. Results: The mean Ki‐67 LI for oral SCCs at the ITF was significantly greater than that for the excision margin tissue ( P < 0.0001). There was a positive association between increasing Ki‐67 LI and increasing Broders' grade ( P < 0.05), with a well‐differentiated tumour having the lowest mean Ki‐67 LI (1549 ± 806) and a poorly differentiated tumour having the highest value (2232 ± 771). A similar trend was observed between the mean Ki‐67 LI and Bryne's multifactorial grading system. Conclusions: It was concluded from this study that cell proliferation (as measured by the Ki‐67 antigen) at the ITF had a strong positive relationship with histological grading in human oral SCC.