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Diffusion of reduced arecoline and arecaidine through human vaginal and buccal mucosa
Author(s) -
Bijl P.,
Eyk A. D.,
Wyk C. W.,
Stander I. A.
Publication year - 2001
Publication title -
journal of oral pathology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.887
H-Index - 83
eISSN - 1600-0714
pISSN - 0904-2512
DOI - 10.1034/j.1600-0714.2001.300402.x
Subject(s) - arecoline , oral mucosa , buccal mucosa , medicine , areca , pathology , dentistry , receptor , muscarinic acetylcholine receptor , oral cavity , structural engineering , nut , engineering
Because alkaloids from areca nut, arecoline and arecaidine, have been implicated in the development of oral submucous fibrosis, we determined their diffusion kinetics through human buccal and vaginal mucosa. Four clinically healthy vaginal mucosa specimens (mean patient age± standard deviation: 47±15 years; age range: 31–60 years) and 4 buccal mucosa specimens from 2 male patients and 2 female patients (mean patient age±standard deviation: 31±9 years; age range: 17–53 years) were obtained during surgery. In vitro flux rates of reduced arecoline and arecaidine (r‐arecoline and r‐arecaidine) were determined by use of a flow‐through diffusion apparatus. Analysis of variance, a Duncan multiple range test, and an unpaired t ‐test were used to determine steady state kinetics and flux differences over time intervals. Although statistically significant differences were observed between flux values for both alkaloids and tissues at certain time points, these were not considered to be of biological (clinical) significance. However, the flux rates across both mucosa of r‐arecoline were significantly higher statistically than those of r‐arecaidine. The findings demonstrated the differences in the diffusion kinetics between r‐arecoline and r‐arecaidine across human buccal and vaginal mucosa, an observation that could be explained in terms of their ionisation characteristics. Additionally, the results obtained further support the hypothesis that human vaginal mucosa can be used as a model for buccal mucosa in studies of permeability to various chemical compounds.