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Enhancing effects of fibroblast growth factor on the proliferation of salivary gland carcinoma cells and salivary gland carcinogenesis
Author(s) -
Yura Yoshiaki,
Yoshioka Yukio,
Yamamoto Sin,
Kusaka Jun,
Bando Takashi,
Yoshida Hideo,
Sato Mitsunobu
Publication year - 2001
Publication title -
journal of oral pathology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.887
H-Index - 83
eISSN - 1600-0714
pISSN - 0904-2512
DOI - 10.1034/j.1600-0714.2001.300306.x
Subject(s) - submandibular gland , carcinogenesis , salivary gland , immunohistochemistry , biology , endocrinology , medicine , dmba , pathology , cancer , immunology
The proliferation of mouse submandibular gland carcinoma YT‐12 cells was stimulated by endothelial cell growth factor (ECGF)/bovine brain‐derived acidic fibroblast growth factor (aFGF) and recombinant human aFGF. To determine whether aFGF was capable of modifying salivary gland carcinogenesis, the effect of brain‐derived aFGF was examined in vivo . Mice in Groups 1 and 2 were injected with 9,10‐dimethyl‐1,2‐benzanthracene (DMBA) into the left submandibular gland, and then Group 1 mice received bovine brain‐derived aFGF and Group 2 mice received vehicle subcutaneously for 10 weeks. Group 3 and 4 mice received either bovine brain‐derived aFGF or vehicle only. Sixteen weeks after the start of the experiment, the incidence of submandibular gland carcinomas in Group 1 was significantly greater than that in Group 2. Immunohistochemical study indicated that ducts in the normal submandibular glands and carcinomas showed positive staining with anti‐aFGF antibody. Immunoblot and reverse transcriptase‐polymerase chain reaction (RT‐PCR) analysis revealed the expression of aFGF in these tissues. FGF receptor (FGFR)‐1 and FGFR‐4 were detectable in the mouse submandibular glands and carcinomas. These findings suggest that bovine brain‐derived aFGF stimulates the proliferation of submandibular gland carcinoma cells and promotes mouse submandibular gland carcinogenesis.