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Immune competent cells in non‐irradiated autologous jejunal grafts used for reconstruction of the oral cavity and oropharynx
Author(s) -
Millesi Werner,
Formanek Michael,
Moser Doris,
Kornfehl Johannes
Publication year - 2001
Publication title -
journal of oral pathology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.887
H-Index - 83
eISSN - 1600-0714
pISSN - 0904-2512
DOI - 10.1034/j.1600-0714.2001.300205.x
Subject(s) - antigen , immune system , immunohistochemistry , monoclonal antibody , biopsy , cd19 , pathology , transplantation , antibody , medicine , biology , immunology
Autologous jejunal grafts used for primary reconstruction in cases of extensive soft tissue defects following tumor resection in the upper aerodigestive tract were investigated by immunhistochemistry (APAAP technique). Biopsies from eight patients were taken intra‐operatively, at the time of transplantation and 2, 4, and 6 months post‐operatively. A panel of monoclonal antibodies directed against surface antigens of the major subpopulations of the immune system was used. In all of the patients, a remarkable increase of all antigens investigated was detected 2 months post‐operatively, which remained unchanged in the following biopsies (4 and 6 months postoperatively). Significantly higher numbers of CD45RA + and CD45RO + ( P <0.05) leukocytes were detectable. This increase was due to both subsets of T and B cells, but only for CD19 + B cells was the increase significant. In addition, NK cells (CD16 + lymphocytic cells, P <0.01) and mature macrophages (25F9 + cells, P <0.01) increased. The first post‐operative biopsy showed a significantly higher expression of activation‐associated antigens (ICAM‐1, VCAM, and HLA‐DR) on monocytes/macrophages and endothelial cells. Our findings indicate that autologous jejunal grafts facilitate immunological function in the new microenvironment.