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Enamel proteins and extracellular matrix molecules are co ‐localized in the pseudocystic stromal space of adenomatoid odontogenic tumor
Author(s) -
Murata Masashi,
Cheng Jun,
Horino Kazuhito,
Hara Kohji,
Shimokawa Hitoyata,
Saku Takashi
Publication year - 2000
Publication title -
journal of oral pathology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.887
H-Index - 83
eISSN - 1600-0714
pISSN - 0904-2512
DOI - 10.1034/j.1600-0714.2000.291002.x
Subject(s) - adenomatoid odontogenic tumor , ameloblast , extracellular matrix , pathology , laminin , stromal cell , chemistry , fibronectin , anatomy , microbiology and biotechnology , biology , enamel paint , medicine , ameloblastoma , maxilla , dentistry
In order to examine the functional differentiation of tumor cells of adenomatoid odontogenic tumor (AOT) as ameloblasts and to determine the participation of the extracellular matrix (ECM) in the formation of its characteristic histologic architecture, tissue samples from five cases of adenomatoid odontogenic tumor were examined by immunohistochemical staining for enamel proteins and ECM molecules. Amelogenin, enamelin, laminin, heparan sulfate proteoglycan, fibronectin, collagen type IV and type V were immunolocalized within the luminal space and along the inner rim of duct‐like structures. Eosinophilic hyaline droplets within the whorled or rosette masses of tumor cells showed basically the same staining pattern as the luminal contents. High columnar tumor cells that formed duct‐like structures were immunopositive for amelogenin, while the staining intensity decreased with flattening of the cells, which was a result of luminal growth. The findings suggest that the constituent cells of duct‐like structures are differentiated once to ameloblasts but fail to mature further due instead to increased production of ECM molecules and due to their retention in the lumina. It is possible to regard these special structures in AOT as stromal pseudocysts.