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Immunohistochemical study of the distribution of endogenous biotin and biotin‐binding enzymes in ductal structures of salivary gland tumours
Author(s) -
Lu ChangSheng,
Kashima Kenji,
Daa Tsutomu,
Yokoyama Shigeo,
Yanagisawa Shigetaka,
Nakayama Iwao
Publication year - 2000
Publication title -
journal of oral pathology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.887
H-Index - 83
eISSN - 1600-0714
pISSN - 0904-2512
DOI - 10.1034/j.1600-0714.2000.290905.x
Subject(s) - biotin , pleomorphic adenoma , adenoid cystic carcinoma , myoepithelial cell , biology , mucoepidermoid carcinoma , immunohistochemistry , salivary gland , pathology , ductal cells , adenocarcinoma , carcinoma , pyruvate carboxylase , enzyme , medicine , biochemistry , cancer , genetics
To clarify the pathologic value of endogenous biotin in the salivary gland, we examined in a series of neoplasms of the salivary gland by immunohistochemical staining the distribution of endogenous biotin and of biotin‐binding enzymes, namely, acetyl CoA carboxylase (AC), which is a cytosolic enzyme, and pyruvate carboxylase (PC), which is a mitochondrial enzyme. In pleomorphic adenoma, we found biotin and PC in ductal epithelial elements, while AC was found mainly in myoepithelial elements. Carcinoma ex pleomorphic adenoma, adenocarcinoma and mucoepidermoid carcinoma were frequently immunopositive for biotin, PC and AC, while adenoid cystic carcinoma was rarely immunopositive for biotin, PC or AC. These results indicate that endogenous biotin might be associated with the mitochondrial enzyme, which is present at high levels in ductal cells of the salivary gland. However, the neoplastic cells in adenoid cystic carcinoma seemed to have an unusual expression of biotin and related enzymes.