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Dissemination of cancer cells into circulation occurs by incisional biopsy of oral squamous cell carcinoma
Author(s) -
Kusukawa Jingo,
Suefuji Yuichi,
Ryu Fuminori,
Noguchi Ryo,
Iwamoto Osamu,
Kameyama Tadamitsu
Publication year - 2000
Publication title -
journal of oral pathology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.887
H-Index - 83
eISSN - 1600-0714
pISSN - 0904-2512
DOI - 10.1034/j.1600-0714.2000.290703.x
Subject(s) - medicine , biopsy , incisional biopsy , cancer , cytokeratin , metastasis , pathology , carcinoma , basal cell , immunohistochemistry
To examine whether cancer cell dissemination results from incisional biopsy, we tried to detect squamous cell carcinoma (SCC) cells in peripheral blood before and after incisional biopsy by means of cytokeratin 19 (CK19) reverse‐transcriptase polymerase chain reaction (RT‐PCR). The study population consisted of 20 patients with oral SCC; 10 were given incisional biopsies followed by radical excision (the incisional biopsy group), and the remaining 10 were treated by excisional biopsy alone (the excisional biopsy group). Ten non‐oral cancer patients with benign oral lesions served as controls. Five‐ml blood aspirates collected before and after incision were used for CK19 RT‐PCR. Two (20.0%) of 10 patients from the incisional biopsy group were positive for CK19 transcripts in their peripheral blood drained 15 min after incision. In contrast, CK19 transcript was not detected either in the excisional biopsy group or in controls. Surgical invasiveness for oral cancer, including incisional biopsy, causes dissemination of cancer cells into circulation, resulting in increased risk of metastasis.