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Biotypes of oral Candida albicans isolated from AIDS patients and HIV‐free subjects in Thailand
Author(s) -
Teanpaisan R.,
Nittayananta W.,
Chongsuvivatwong V.
Publication year - 2000
Publication title -
journal of oral pathology and medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.887
H-Index - 83
eISSN - 1600-0714
pISSN - 0904-2512
DOI - 10.1034/j.1600-0714.2000.290501.x
Subject(s) - candida albicans , ketoconazole , corpus albicans , amphotericin b , microbiology and biotechnology , biology , mycosis , minimum inhibitory concentration , fungi imperfecti , antifungal , immunology , antimicrobial
This study was conducted to examine biotypes and antifungal susceptibility patterns of oral Candida albicans isolated from HIV‐infected patients, HIV‐free patients with candidiasis and healthy subjects. All isolates were biotyped using a typing system based on enzyme profiles, carbohydrate assimilation patterns and boric acid resistance. Thirty‐eight biotypes were found amongst 218 oral C. albicans isolates. The major biotype found was A1S, which accounted for 32.6% of all isolates, and this biotype was the most common in all groups. There was a greater variety of biotypes of C. albicans in the HIV‐infected group than in the other groups; however, there was no statistically significant difference between the groups. The minimum inhibitory concentrations (MICs) of a total of 118 isolates were determined for amphotericin B and for ketoconazole using the National Committee for Clinical Laboratory Standards (NCCLS) broth macrodilution method and the E‐test. When the antifungal susceptibility patterns among the groups were compared, a statistically significant difference was found only with amphotericin B. The median MIC of amphotericin B in the HIV‐infected group was higher than in the healthy group ( P =0.013, NCCLS method; P =0.002, E‐test). However, this difference in sensitivity was not restricted to any sub‐type investigated. Our results showed that the biotype patterns of C. albicans isolates that colonize HIV‐infected patients are similar to those of HIV‐free subjects, and there is no relationship between antifungal susceptibility patterns and the biotypes.