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Exposure of cynomolgus monkey embryos to retinoic acid causes thymic defects: effects on peripheral lymphoid organ development[Note 1. Funding: This research was supported by NIH grant RR00169. ...]
Author(s) -
Makori N.,
Peterson P.E.,
Lantz K.,
Hendrickx A.G.
Publication year - 2002
Publication title -
journal of medical primatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.31
H-Index - 42
eISSN - 1600-0684
pISSN - 0047-2565
DOI - 10.1034/j.1600-0684.2002.01013.x
Subject(s) - spleen , biology , thymocyte , lymphatic system , white pulp , retinoic acid , lymphocyte , t cell , immunology , red pulp , pathology , cd3 , cd8 , andrology , immune system , medicine , cell culture , genetics
We have previously reported that exposure of monkey embryos to 13‐ cis ‐retinoic acid (cRA) results in thymic defects. In this study, we analyzed lymphocyte and antigen‐presenting cell populations at gestational days (GDs) 80–100 in the thymus, spleen, mesenteric lymph nodes, and gut‐associated lymphoid tissue following a teratogenic dosing regimen of cRA (2.5 and 5 mg/kg) at GD14–27. Tissue sections were immunostained for T‐cells (anti‐CD3), B‐cells (anti‐CD20), dendritic cells (p55), and major histocompatibility class II (anti‐HLA‐DR). Digital images of spleen sections were analyzed to obtain the relative area occupied by the cell subsets within the white pulp (WP). Compared with controls, the T‐cell dependent compartment of the spleen WP in specimens with perturbed thymic development (aplasia and severe hypoplasia) showed a reduction in size and proportion of CD3 + T cells. Our findings indicate that cRA‐induced thymic defects result in disrupted development of the splenic T‐cell dependent compartment.