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Effect of vaccination with recombinant modified vaccinia virus Ankara expressing structural and regulatory genes of SIV macJ5 on the kinetics of SIV replication in cynomolgus monkeys
Author(s) -
Negri Donatella R.M.,
Baroncelli Silvia,
Michelini Zuleika,
Macchia Iole,
Belli Roberto,
Catone Stefania,
Incitti Fabio,
Ten Haaft Peter,
Corrias Franco,
Cranage Martin P.,
Polyanskaya Natasha,
Norley Stephen,
Heeney Jonathan,
Verani Paola,
Titti Fausto
Publication year - 2001
Publication title -
journal of medical primatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.31
H-Index - 42
eISSN - 1600-0684
pISSN - 0047-2565
DOI - 10.1034/j.1600-0684.2001.d01-53.x
Subject(s) - simian immunodeficiency virus , virology , modified vaccinia ankara , vaccination , vaccinia , biology , immunology , virus , antibody , antigen , vector (molecular biology) , recombinant dna , gene , biochemistry
The efficacy of a multicomponent vaccination with modified vaccinia Ankara constructs (rMVA) expressing structural and regulatory genes of simian immunodeficiency virus (SIV mac251/32H/J5 ) was investigated in cynomolgus monkeys, following challenge with a pathogenic SIV. Vaccination with rMVA‐J5 performed at week 0, 12, and 24 induced a moderate proliferative response to whole SIV, a detectable humoral response to all but Nef SIV antigens, and failed to induce neutralizing antibodies. Two months after the last boost, the monkeys were challenged intravenously with 50 MID 50 of SIV mac251 . All control monkeys, previously inoculated with non‐recombinant MVA, were infected by week two and seroconverted by weeks four to eight. In contrast a sharp increase of both humoral and proliferative responses at two weeks post‐challenge was observed in vaccinated monkeys compared to control monkeys. Although all vaccinated monkeys were infected, vaccination with rMVA‐J5 appeared to partially control viral replication during the acute and late phase of infection as judged by cell‐ and plasma‐associated viral load.