Premium
Immunolocalization of proliferating cell nuclear antigen (PCNA) in cynomolgus monkey ( Macaca fascicularis ) testes during postnatal development
Author(s) -
Liang JiHong,
Sankai Tadashi,
Yoshida Takashi,
Yoshikawa Yasuhiro
Publication year - 2001
Publication title -
journal of medical primatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.31
H-Index - 42
eISSN - 1600-0684
pISSN - 0047-2565
DOI - 10.1034/j.1600-0684.2001.300206.x
Subject(s) - proliferating cell nuclear antigen , sertoli cell , biology , leydig cell , immunohistochemistry , medicine , spermatogenesis , testicle , endocrinology , antigen , proliferation marker , andrology , immunology , hormone , luteinizing hormone
The age‐related distribution of proliferating cell nuclear antigen (PCNA) in the testes of cynomolgus monkeys ( Macaca fascicularis ) during postnatal development was detected using light‐microscopic immunohistochemistry. In neonatal testes, some PCNA‐positive spermatogonia, Sertoli cells, peritubular cells, and Leydig cells were detected. In early infantile testes, only a few of these cell types were positive. In late infantile testes, the numbers of positive cells were greater than in the earlier developmental stages. In pubertal testes, the numbers of positive spermatogonia, spermatocytes, Sertoli cells, peritubular cells, and Leydig cells were considerably higher. In adult testes, a larger percentage of spermatogonia and spermatocytes was positive, and peritubular cells and Leydig cells were occasionally positive; secondary spermatocytes, spermatids, and Sertoli cells were not positive. We concluded that immunolocalization of PCNA can serve as a tool for studying proliferation status in developing testes of cynomolgus monkeys. A relatively low proliferative activity in early infantile testes and a remarkable increase of proliferative activity in pubertal testes correlate with the fluctuations of steroidogenic functions during postnatal development in cynomolgus monkeys.