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Routine DNA analysis based on 12S rRNA gene sequencing as a tool in the management of captive primates
Author(s) -
Van Der Kuyl Antoinette C.,
Van Gennep David R.O.,
Dekker John T.,
Goudsmit Jaap
Publication year - 2000
Publication title -
journal of medical primatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.31
H-Index - 42
eISSN - 1600-0684
pISSN - 0047-2565
DOI - 10.1034/j.1600-0684.2000.290501.x
Subject(s) - mitochondrial dna , phylogenetic tree , biology , primer (cosmetics) , primate , gene , polymerase chain reaction , genetics , dna sequencing , dna , ribosomal rna , phylogenetics , 16s ribosomal rna , evolutionary biology , ecology , chemistry , organic chemistry
Automated DNA sequencing of a fragment of the relatively slowly evolving mitochondrial 12S rRNA gene was used to distinguish primate species, and the method was compared with species determination based upon classical taxonomy. DNA from blood from 53 monkeys housed at the Stichting AAP Shelter for Exotic Animals, all Old World monkeys, was amplified by polymerase chain reaction (PCR) with a primer set spanning approximately 390 nucleotides of the mitochondrial 12S rRNA gene. The products were directly sequenced and compared with our database of primate 12S sequences. Many individuals were found to harbor a 12S sequence identical to one of the reference sequences. For others, phylogenetic methods were used for species estimation, which was especially informative in Cercopithecus species.