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Expression of claudin‐5 in dermal vascular endothelia
Author(s) -
Morita Kazumasa,
Sasaki Hiroyuki,
Furuse Kyoko,
Furuse Mikio,
Tsukita Shoichiro,
Miyachi Yoshiki
Publication year - 2003
Publication title -
experimental dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.108
H-Index - 96
eISSN - 1600-0625
pISSN - 0906-6705
DOI - 10.1034/j.1600-0625.2003.120309.x
Subject(s) - claudin , microbiology and biotechnology , tight junction , biology , chemistry
Claudins and occludin are integral membrane proteins at tight junctions (TJs). We examined subcellular localization of claudin‐5 and occludin in dermal vascular endothelia. Immunofluorescence staining showed that claudin‐5 was expressed at the cell–cell border of dermal vascular endothelia in mouse skin. However, in some dermal vessels, claudin‐5 expression was markedly decreased or absent in amount by double‐immunofluorescence stainings with PECAM‐1 and PAL‐E. In contrast, occludin was not detected in dermal vessels. Freeze‐fracture and immunoreplica electron microscopy on primary‐cultured human dermal endothelial cells showed that claudin‐5 was localized at tight junctions. These findings confirmed that TJs in dermal vascular endothelial cells are composed of claudin‐5.