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Decreased IL‐10 production by psoriatic peripheral blood mononuclear cells stimulated with streptococcal superantigen
Author(s) -
Aiba Setsuya,
Uddin Zia,
Nakagawa Satoshi,
Sugawara Syunji,
Rikiishi Hidemi,
Kumagai Katsuo,
Tagami Hachiro
Publication year - 2002
Publication title -
experimental dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.108
H-Index - 96
eISSN - 1600-0625
pISSN - 0906-6705
DOI - 10.1034/j.1600-0625.2002.110407.x
Subject(s) - superantigen , peripheral blood mononuclear cell , immunology , psoriasis , medicine , streptococcus pyogenes , antigen , cytokine , staphylococcus aureus , biology , t cell , immune system , biochemistry , genetics , bacteria , in vitro
The strong association of acute guttate psoriasis and streptococcal throat infection has suggested a role for streptococcal antigens in the pathogenesis of psoriasis. We have reported that psoriatic peripheral blood mononuclear cells (PBMCs) showed significantly lower responses to cytoplasmic membrane‐associated protein (CAP) isolated from group A β‐hemolytic streptococci, a kind of streptococcal superantigen. The objectives were to evaluate the abnormal cytokine production by psoriatic PBMCs to streptococcal superantigen, CAP. We compared the production of four different cytokines, i.e. IL‐4, IL‐5, IL‐10, and IFN‐γ, by PBMCs between psoriatic patients and healthy controls after stimulation with CAP or two different staphylococcal superantigens, staphylococcal enterotoxin A (SEA) or E (SEE). When PBMCs were stimulated with CAP, the production of IL‐10 was significantly lower by psoriatic PBMCs than by those from healthy controls, whereas those of IL‐4, IL‐5, or IFN‐γ were not different between the two groups. Such a significant decrease in IL‐10 production by psoriatic PBMCs was not observed when they were stimulated with staphylococcal superantigens. Flow cytometric analysis of intracytoplasmic IL‐10 demonstrated defective IL‐10 production by psoriatic PBMCs in both CD3+ T cells and CD14+ monocytes. There was a significant positive correlation between IFN‐γ production by PBMCs and the proliferation of Vβ8+ T cells preferentially stimulated by CAP. These data demonstrating the defective IL‐10 production by psoriatic PBMCs stimulated with streptococcal superantigen seem to explain why only psoriatic patients evolve sustained and Th‐1 deviated skin lesions after streptococcal upper respiratory infection.