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The nitroxide Tempol affords protection against ultraviolet radiation in a transgenic murine fibroblast culture model of cutaneous photoaging
Author(s) -
Bernstein E. F.,
Kong S. K.,
Brown D. B.,
Kwak B. C.,
Takeuchi T.,
Gasparro F. P.,
Uitto J.
Publication year - 2001
Publication title -
experimental dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.108
H-Index - 96
eISSN - 1600-0625
pISSN - 0906-6705
DOI - 10.1034/j.1600-0625.2001.100107.x
Subject(s) - photoaging , superoxide dismutase , reactive oxygen species , chemistry , elastin , ultraviolet , in vitro , pharmacology , biochemistry , biophysics , oxidative stress , dermatology , biology , medicine , materials science , pathology , optoelectronics
The generation of reactive oxygen species is among the various mechanisms by which ultraviolet radiation damages skin. Tempol, a superoxide dismutase analogue which readily penetrates cell membranes when administered exogenously, has been shown to provide protection against some forms of oxygen‐dependent damage. In this study, we measured the ability of Tempol to protect against ultraviolet A‐ and ultraviolet B‐induced damage, using a previously described transgenic mouse model of cutaneous photoaging. The ability of Tempol to prevent ultraviolet radiation‐induced elastin promoter activation was determined in vitro . Tempol provided over 50% protection against ultraviolet B and over 70% protection against ultraviolet A as measured in our in vitro system. These results demonstrate the ability of the superoxide dismutase mimic, Tempol, to protect against ultraviolet induced elastin promoter activation. This compound could be a useful pharmacological agent to prevent cutaneous photoaging.