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Epidermal CD8 + T cells in chronic plaque psoriasis are Tc1 cells producing heterogeneous levels of interferon‐gamma
Author(s) -
Ovigne J.M.,
Baker B. S.,
Brown D. W.,
Powles A. V.,
Fry L.
Publication year - 2001
Publication title -
experimental dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.108
H-Index - 96
eISSN - 1600-0625
pISSN - 0906-6705
DOI - 10.1034/j.1600-0625.2001.010003168.x
Subject(s) - cd8 , cytotoxic t cell , interferon gamma , cytokine , t cell , microbiology and biotechnology , interleukin 2 , biology , interferon , interleukin , immunology , chemistry , in vitro , immune system , biochemistry
The majority of epidermal CD8 + T cells in chronic plaque psoriasis are activated Tc1 cells producing interferon‐γ and no interleukin‐4, a small proportion of which express NK‐T receptors. To quantitate their level of cytokine production and characterize them further, CD8 + T cells were isolated from epidermal cell suspensions of lesional biopsies from 24 patients with chronic plaque psoriasis. T‐cell lines (TCL) were established by culture of CD8 + T cells with feeders and IL‐2 for 11 days and expansion with PHA. Ten TCL were stained for surface markers; 6 were cloned with PHA by limiting dilution. Interferon‐γ, interleukin‐4 and interleukin‐10 production was measured by ELISA after PMA/anti‐CD3 activation of 15 TCL and 39 CD8 + T‐cell clones. The 10 TCL stained were CD8αβ + (93.3%), T‐cell receptor‐αβ + (99.5%), costimulatory molecule CD28 + (90.1%), with a small CD8αα + population (2.3%). No NK‐T‐cell receptor CD158a or CD158b expression was detected, whilst CD94 was expressed on 6.2% of cells in 6/9 TCL. All the TCL and 37/39 CD8 + T‐cell clones produced interferon‐γ but no or minimal interleukin‐4 or interleukin‐10. The TCL produced a wide range of interferon‐γ levels (138 to 15,020 pg/ml). Clones from 3 patients showed low levels (60 to 1,410 pg/ml), from 2 patients high levels (6,105 to 43,040 pg/ml) and from 1 patient a wide range (405 to 36,010 pg/ml) of interferon‐γ production. Thus epidermal CD8 + Tc1 cells in chronic plaque psoriasis produce highly heterogeneous levels of interferon‐γ, which may reflect clinical diversity.