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VEGF production, cell proliferation and apoptosis of human IGR 1 melanoma cells under nIFN‐α/β and rIFN‐γ treatment
Author(s) -
Bölling Brigitte,
Fandrey Joachim,
Frosch Peter J.,
Acker Helmut
Publication year - 2000
Publication title -
experimental dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.108
H-Index - 96
eISSN - 1600-0625
pISSN - 0906-6705
DOI - 10.1034/j.1600-0625.2000.009005327.x
Subject(s) - apoptosis , cell growth , vascular endothelial growth factor , melanoma , cell culture , cancer research , dna synthesis , angiogenesis , biology , medicine , endocrinology , in vitro , vegf receptors , biochemistry , genetics
The effect of natural and recombinant interferons (nIFN, rIFN) on cell growth, apoptosis and the production of vascular endothelial growth factor (VEGF) was investigated in the human melanoma cell line IGR 1. We determined cell proliferation, cell vitality, DNA synthesis, apoptosis, intracellular oxygen radicals (ROS) and VEGF‐mRNA as well as VEGF‐protein levels. rIFN‐γ significantly inhibited growth by decreasing DNA synthesis and increasing apoptosis. Less pronounced was the growth inhibitory effect of nIFN‐β because an increased rate of apoptosis was outweighed by enhanced DNA synthesis. nIFN‐α only had minor effects on cell growth parameters. Under long‐term incubation (144 h) nIFN‐β decreased, but rIFN‐γ increased production of the angiogen VEGF. Our data underscore the multiple effects of IFNs on melanoma cells and may contribute to the understanding of ambivalent results of melanoma therapy by IFNs. Particularly, the increased VEGF production under long‐term treatment with serum IFN levels between 100 and 1200 IU/ml should be kept in mind.