Premium
A three‐dimensional skin culture model for mouse keratinocytes: application to transgenic mouse keratinocytes
Author(s) -
Carroll J. M.,
Molès J.P.
Publication year - 2000
Publication title -
experimental dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.108
H-Index - 96
eISSN - 1600-0625
pISSN - 0906-6705
DOI - 10.1034/j.1600-0625.2000.009001020.x
Subject(s) - epidermis (zoology) , keratinocyte , genetically modified mouse , transgene , dermis , microbiology and biotechnology , biology , in vitro , in vivo , integrin , cell , anatomy , genetics , gene
The study of mouse epidermal biology has been hampered by the lack of a good in vitro model for the culture of mouse keratinocytes which allowed the reconstruction of a fully differentiated epidermis. We adapted the Pruniéras' model, also called the Dead de‐Epidermized Dermis model (DED), to mouse keratinocytes and showed that a neo‐epidermis can be reconstructed exhibiting a complete differentiation program. We also used this model to culture transgenic mouse keratinocytes. We observed that transgene expression occurred in the correct location and that the neo‐epidermis mimed previous in vivo observations obtained with integrin skin‐targeted transgenic mice. Therefore, this model will be a powerful tool to further investigate normal mouse and transgenic keratinocyte biology.