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Expression of genes regulating angiogenesis in human circulating hematopoietic cord blood CD34+/CD133+ cells
Author(s) -
Pomyje Jiří,
Živný Jan,
Šefc Luděk,
Plašilová Magdalena,
Pytlík Robert,
Nečas Emanuel
Publication year - 2003
Publication title -
european journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0902-4441
DOI - 10.1034/j.1600-0609.2003.00040.x
Subject(s) - cd34 , cord blood , haematopoiesis , angiogenesis , biology , bone marrow , population , stem cell , microbiology and biotechnology , immunology , cancer research , medicine , environmental health
Objectives: Human CD34+ cells represent a heterogeneous population of immature cells which may differentiate to various cell types. The aim of the study was to determine angiogenesis regulating genes expression in CD34+ cells, their subpopulations, and during their differentiation induced by hematopoietic growth factors. Material and methods: We have measured the expression of angiogenesis regulating genes angiopoietin‐1 (Ang‐1), angiopoietin‐1 (Ang‐2) and their receptor Tie‐2, vascular endothelial growth factor (VEGF) and its receptors VEGFR‐1 and VEGFR‐2 in sorted population of CD34+ and CD34+/CD133+ cells from human cord blood and bone marrow, and in their differentiating progeny, using real time reverse transcriptase polymerase chain reaction. The hematopoietic differentiation of CD34+ cells was induced in semisolid or liquid differentiation supporting media containing appropriate hematopoietic growth factors. Results: A higher expression of Ang‐1, Ang‐2, and Tie‐2 mRNAs was detected in CD34+/CD133+ cord blood cells as compared with CD34−/CD133− fraction, but no expression of these genes was detected in burst‐forming unit erythroid (BFU‐E) nor colony‐forming unit granulocyte macrophage (CFU‐GM) colonies. The level of Ang‐1 and Tie‐2 mRNAs, but not that of Ang‐2 mRNA gradually decreased during a 14‐d incubation of cord blood CD34+ cells in a liquid culture. A significantly higher expression of VEGF mRNA was in BFU‐E as compared with CFU‐GM cell colonies and CD34+/CD133+ cells. VEGFR‐1 mRNA was equally expressed in CD34+/CD133+ and CD34−/CD133− cells as well as in BFU‐E and CFU‐GM colonies. Expression of VEGFR‐2 mRNA was detected at the borderline of method sensitivity only in CD34+/CD133+ cells. Conclusion: CD34+/CD133+ cord blood cells express Ang‐1, Ang‐2 and VEGF as well as their receptor mRNAs, suggesting a role of these cells in regulation both angiopoiesis and hematopoiesis.