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Decreased amount of mpl and reduced expression of glycoprotein IIb/IIIa and glycoprotein Ib on platelets from patients with refractory anemia: analysis by a non‐isotopic quantitative ligand binding assay and immunofluorescence
Author(s) -
Izumi Masakazu,
Takeshita Akihiro,
Shinjo Kaori,
Naito Kensuke,
Matsui Hirotaka,
Shibata Kiyoshi,
Ohnishi Kazunori,
Kanno Takashi,
Ohno Ryuzo
Publication year - 2001
Publication title -
european journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0902-4441
DOI - 10.1034/j.1600-0609.2001.066004245.x
Subject(s) - platelet , chemistry , platelet membrane glycoprotein , ligand (biochemistry) , monoclonal antibody , microbiology and biotechnology , immunofluorescence , ligand binding assay , glycoprotein , medicine , immunology , antibody , receptor , biochemistry , biology
Using a non‐isotopic ligand binding assay and immunofluorescence, we examined the amount of mpl , glycoprotein IIb/IIIa (gpIIb/IIIa) and glycoprotein Ib (gpIb) on platelets from healthy volunteers and patients with refractory anemia (RA). For the analysis of mpl expression, we applied both a non‐isotopic ligand binding assay and immunofluorescence using anti‐ mpl monoclonal antibody, and compared the results from both methods. The non‐isotopic ligand binding assay has been developed in our laboratory and is suitable for the quantitative analysis of a small amount of cytokine receptors such as mpl on platelets. In platelets from patients with RA, the amount of mpl expressed by the D value was 0.05±0.03 (mean±standard deviation), and was significantly lower than that in healthy volunteers (0.15±0.05, p <0.0001). The mean fluorescence intensities (MFI) of gpIIb/IIIa and gpIb on platelets from RA patients were 28.8±8.8 and 20.8±7.7, respectively, and were significantly lower than those on normal subjects (93.2±22.6 and 67.4±9.1, p <0.0001 and p <0.0001, respectively). There was a good correlation between the amount of mpl and the MFI of gpIIb/IIIa ( p =0.794, p <0.0001) or gpIb ( p =0.774, p <0.0001), and between those of gpIIb/IIIa and gpIb ( p =0.728, p <0.0001). We demonstrated a decreased amount of mpl as well as a reduced expression of gpIIb/IIIa and gpIb on platelets from RA patients.