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Growth factor receptor profile of CD34 + cells in AML and B‐lineage ALL and in their normal bone marrow counterparts
Author(s) -
De Waele M.,
Renmans W.,
Vander Gucht K.,
Jochmans K.,
Schots R.,
Otten J.,
Trullemans F.,
Lacor P.,
Van Riet I.
Publication year - 2001
Publication title -
european journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0902-4441
DOI - 10.1034/j.1600-0609.2001.00320.x
Subject(s) - cd34 , bone marrow , clonogenic assay , myeloid , biology , receptor , stem cell factor , cancer research , haematopoiesis , stem cell , growth factor , microbiology and biotechnology , immunology , cell , biochemistry
Leukaemic cells show a low clonogenic activity and a heterogeneous proliferative response to growth factors. We investigated whether this could be due to an altered expression of growth factor receptors on the leukaemic precursors. Receptors for G‐CSF, stem cell factor (SCF), IL‐3, IL‐6 and IL‐7 were detected on CD34 + cells in AML and B‐lineage ALL with monoclonal antibodies and flow cytometry. The expression was compared with that on myeloid and B‐lymphoid CD34 + cells in normal bone marrow. Leukaemic CD34 + cells expressed the same receptors as their normal counterparts. AML and B‐lineage ALL could be distinguished by the growth factor receptor profile of their CD34 + cells. SCFR, G‐CSFR and IL‐6Rα were found in AML, IL‐7R in B‐lineage ALL and IL‐3Rα in both. IL‐3Rα was upregulated in AML and B‐lineage ALL CD34 + cells, while samples with low or high expression were present for the other receptors. This variable expression could correlate with the heterogeneous response of leukaemic cells to growth factors. Functional studies on isolated CD34 + cells are needed to investigate this further.