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Granulocytopoiesis versus cytokine activities in peritoneal diffusion chamber cultures in the mouse
Author(s) -
Wang Xiuli,
Brekken Kristin B.,
Siebke ElseMarie,
Stokke Gro,
Sørlid Hanne K.,
Benestad Haakon B.
Publication year - 2000
Publication title -
european journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0902-4441
DOI - 10.1034/j.1600-0609.2000.90070.x
Subject(s) - granulocyte , progenitor cell , population , cytokine , haematopoiesis , granulocyte macrophage colony stimulating factor , tumor necrosis factor alpha , immunology , endocrinology , medicine , colony stimulating factor , cyclophosphamide , andrology , biology , stem cell , microbiology and biotechnology , chemotherapy , environmental health
Regulation of granulocyte formation was studied by correlating granulocytopoiesis in diffusion chambers with activities of putative regulatory cytokines in such chambers. The implantation procedure increased the levels in 1‐ and 2‐d chambers of interleukin 6 (IL‐6), G‐CSF, TNF‐α, and non‐specific granulocyte/macrophage (G/M) colony‐stimulating activities (CSA), assessed with bioassays and immunoassays. The activities subsided rapidly thereafter. They could be increased by vinblastine, cyclophosphamide, and a sterile inflammatory reaction (s.c. implanted copper rods; Cu‐r). Anti‐inflammatory indomethacin curtailed the IL‐6, but raised the TNF‐α, G‐CSF, and CSA levels in Cu‐r mice. Interferon inducer poly‐I:C augmented G‐CSF, but decreased TNF‐α levels. Mouse blood cells cultured in chambers expanded their granulocyte/macrophage progenitor population rapidly during the first week of culture; this population being significantly larger on day 3 in perturbed than in unperturbed mice. A marked decline followed in the second week, significantly larger in mice given cytotoxic treatment than in controls. Peritoneal G‐CSF and TNF‐α may explain progenitor and granulocyte growth and development in diffusion chambers during the first week of culture. GM‐CSF and IL‐3 were apparently without any influence. None of the peritoneal cytokines assayed could explain the population decline during the second week, which was possibly caused by exhaustion of earlier progenitor cells, rather than by intra‐chamber feedback mechanisms.