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CD 34 + cells derived from fetal liver contained a high proportion of immature megakaryocytic progenitor cells
Author(s) -
Ma DongChu,
Sun YingHui,
Zuo Wei,
Chang KuiZhong,
Chu JunJie,
Liu YaGe
Publication year - 2000
Publication title -
european journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0902-4441
DOI - 10.1034/j.1600-0609.2000.90038.x
Subject(s) - megakaryocyte , thrombopoietin , biology , haematopoiesis , microbiology and biotechnology , progenitor cell , cd34 , bone marrow , fetus , andrology , immunology , stem cell , medicine , pregnancy , genetics
Endoreplication and maturation of the megakaryocyte (MK) may be retarded or delayed during ontogenesis. In this study, CD 34 + cells were isolated from both human fetal liver and adult bone marrow and incubated with thrombopoietin (TPO). The cell number, morphological characteristics, platelet‐associated antigen phenotype, maturation stage and DNA ploidy of CD 41 + cells were examined from day 0 to day 12 in culture. 1) TPO stimulated the proliferation of fetal liver (FL)‐derived CD 34 + cells with a mean 73.14‐fold increase of CD 41 + cells after 12 d in culture. Adult BM‐derived CD 34 + cells increased only slightly, with a mean 8.18‐fold increase of CD 41 + cells. 2) Although the membrane phenotype of both FL CD 34 + ‐derived MKs and BM CD 34 + ‐derived MKs analyzed with CD 41a , CD 42a , CD 61 and CD 34 were similar, all FL CD 34 + ‐derived MKs were in maturation stage I and II and in low ploidy (<4N) class. By comparison, BM CD 34 + MKs possessed 15% MKs in maturation stage III and IV and with 23% MKs in high ploidy class (>4N). 3) Most of cultured FL‐derived CD 34 + cells did not have a well developed demarcation system (DM) and numerous α‐granules after 12 d incubation. von Willebrand factor (vWF) appeared earlier on the cultured BM‐derived CD 34 + cells than on FL‐derived CD 34 + cells. 4) The expression of both cyclin E and cyclin B 1 progressively increased in FL CD 34 + cells induced by TPO during 12 d in culture. 5) The expression of cyclin D 1 gradually decreased in FL CD 34 + cells induced by TPO over 12 d incubation. 6) Immunocytochemical analysis showed that cyclin D 3 was detected only in cytoplasm of cultured FL‐derived CD 34 + cells, whereas in both cytoplasm and nuclei of cultured BM‐derived CD 34 + cells. These data suggest that FL‐derived CD 34 + cells contain a high proportion of immature megakaryocytic progenitor cells. It further suggests that TPO can push these progenitor cells into proliferation by upregulating the expression of cyclins B 1 and E, and drive a high proportion of cells into megakaryocytic lineage.