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Cell kinetic study of normal humanbone marrow hematopoiesis andacute leukemia using 7AAD/PY
Author(s) -
Toba Ken,
Koike Tadashi,
Watanabe Kenichi,
Fuse Ichiro,
Takahashi Masuhiro,
Hashimoto Shigeo,
Takahashi Hidenobu,
Abe Takashi,
Yano Toshio,
Shibazaki Yasuhiko,
Itoh Hiromi,
Aizawa Yoshifusa
Publication year - 2000
Publication title -
european journal of haematology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.904
H-Index - 84
eISSN - 1600-0609
pISSN - 0902-4441
DOI - 10.1034/j.1600-0609.2000.09005.x
Subject(s) - haematopoiesis , cd34 , bone marrow , leukemia , myeloid , biology , stem cell , acute leukemia , cd38 , microbiology and biotechnology , immunology , chemistry , cancer research
We have used the 7AAD/PY method to analyze the cell cycle status of normal human bone marrow hematopoiesis, and found that the cell kinetics differed. There were cells with relatively low levels of RNA in the S‐phase (Type I) and a high level in the S phase (Type II). T‐cells, B‐cells, nucleated red cells and CD34 + /CD19 + early B‐cells in bone marrow were Type I, whereas myelomonocytic subset and CD34 + /CD33 dim + common myeloid cells were Type II. AC133 + /CD38 dim cells, which were thought to be lineage‐marker negative hematopoietic stem cells, had intermediate amounts of RNA in the S‐phase between Type I and II (Type 0). Seventy‐four cases of acute leukemia were also analyzed. Most of the T‐ and B‐ALL cases were found to be Type I, most of the ANLL cases were Type II, and there were 10 cases that were Type 0. These findings yielded fundamental information about normal hematopoiesis and acute leukemia.