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Modulations of nerve growth factor and Bcl‐2 in ultraviolet‐irradiated human epidermis
Author(s) -
Stefanato Catherine M.,
Yaar Mina,
Bhawan Jag,
Phillips Tania J.,
Kosmadaki Maria G.,
Botchkarev Vladimir,
Gilchrest Barbara A.
Publication year - 2003
Publication title -
journal of cutaneous pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.597
H-Index - 75
eISSN - 1600-0560
pISSN - 0303-6987
DOI - 10.1034/j.1600-0560.2003.00065.x
Subject(s) - nerve growth factor , epidermis (zoology) , apoptosis , staining , melanin , human skin , monoclonal antibody , irradiation , in vivo , erythema , pathology , antibody , biology , microbiology and biotechnology , chemistry , immunology , medicine , anatomy , biochemistry , genetics , receptor , physics , nuclear physics
Background: Ultraviolet (UV) irradiation to the skin causes apoptosis of keratinocytes. Melanocytes are more resistant to UV‐induced apoptosis, due, in part, to high levels of antiapoptotic proteins such as Bcl‐2. In vitro studies have shown that nerve growth factor (NGF), a neurotrophic polypeptide, is produced by keratinocytes and exerts a protective role for melanocytes by upregulating Bcl‐2. The purpose of this study was to determine NGF and Bcl‐2 modulations in UV‐irradiated human skin. Methods: Nine volunteers were irradiated with two minimal erythema doses using solar‐simulated UV irradiation. Seventy‐two hours post irradiation, skin biopsies were obtained from irradiated and sun‐protected skin. The skin specimens were stained with anti‐tyrosinase‐related protein‐1 monoclonal antibody IgG2a (Mel‐5), anti‐Bcl‐2 (monoclonal antibody IgG‐kappa), and with anti‐NGF (polyclonal antibody IgG). Results: NGF staining was identified within the cytoplasm of epidermal melanocytes, similar to the staining observed for TRP‐1 and Bcl‐2. While no significant difference in the number of TRP‐1‐ and Bcl‐2‐positive melanocytes was observed between irradiated and non‐irradiated skin within 72 h, the number of NGF‐positive melanocytes decreased significantly, 72 h after UV irradiation (p < 0.024). NGF was also identified within keratinocytes, and while non‐irradiated skin exhibited cytoplasmic NGF staining throughout the epidermis, NGF staining was reduced in the lower epidermal layers after UV irradiation. Conclusions: This is the first in vivo study showing NGF to be present in melanocytes, as well as showing modulations of NGF and Bcl‐2 in melanocytes, following solar‐simulated UV irradiation.