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Modified short‐term guinea pig sensitization tests for detecting contact allergens as an alternative to the conventional test
Author(s) -
Yanagi Masashi,
Hoya Masatoshi,
Mori Masaaki,
Katsumura Yoshio
Publication year - 2001
Publication title -
contact dermatitis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.524
H-Index - 96
eISSN - 1600-0536
pISSN - 0105-1873
DOI - 10.1034/j.1600-0536.2001.044003140.x
Subject(s) - sensitization , patch test , allergic contact dermatitis , adjuvant , potency , contact dermatitis , chemistry , benzocaine , guinea pig , pharmacology , medicine , allergy , immunology , biochemistry , in vitro
The conventional adjuvant and patch test (APT) method of guinea pig sensitization testing was modified in 2 ways, s‐APT and s‐APT(2), in order to shorten the test period. These short‐term test methods consist of 72‐h closed application of test material with intradermal injection of emulsified Freund’s complete adjuvant (E‐FCA) for 1st induction, 48‐h closed application of test material with (s‐APT) or without (s‐APT(2)) intradermal injection of E‐FCA on the 7th day for 2nd induction, and open application on the 14th day for challenge. They were compared with conventional APT by using 8 allergenic chemicals (formaldehyde, nickel sulfate, cobalt sulfate, ethyl‐p‐aminobenzoate (benzocaine), isoeugenol, 2‐mercaptobenzothiazole, 2,4‐dinitrochlorobenzene (DNCB) and 1‐phenylazo‐2‐naphthol (Sudan I)). The short‐term methods gave similar results to those of conventional APT in terms of mean response, sensitization rate and sensitization potency (challenge concentration that induces a mean response equal to 1.0). Thus, our short‐term methods, which are capable of evaluating skin sensitization within 17 days, are sufficiently sensitive to detect potentially hazardous contact allergens.