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Identification of Lilial ® as a fragrance sensitizer in a perfume by bioassay‐guided chemical fractionation and structure‐activity relationships
Author(s) -
Arnau E. Giménez,
Andersen K. E.,
Bruze M.,
Frosch P. J.,
Johansen J. D.,
Menné T.,
Rastogi S. C.,
White I. R.,
Lepoittevin J.P.
Publication year - 2000
Publication title -
contact dermatitis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.524
H-Index - 96
eISSN - 1600-0536
pISSN - 0105-1873
DOI - 10.1034/j.1600-0536.2000.043006351.x
Subject(s) - bioassay , fractionation , chemistry , identification (biology) , chemical fractionation , chromatography , organic chemistry , biology , botany , genetics
Fragrance materials are among the most common causes of allergic contact dermatitis. The aim of this study was to identify in a perfume fragrance allergens not included in the fragrance mix, by use of bioassay‐guided chemical fractionation and chemical analysis/structure‐activity relationships (SARs). The basis for the investigation was a 45‐year‐old woman allergic to her own perfume. She had a negative patch test to the fragrance mix and agreed to participate in the study. Chemical fractionation of the perfume concentrate was used for repeated patch testing and/or repeated open application test on the pre‐sensitized patient. The chemical composition of the fractions giving a positive patch‐test response and repeated open application test reactions was obtained by gas chromatography‐mass spectrometry. From the compounds identified, those that contained a “structural alert” in their chemical structure, indicating an ability to modify skin proteins and thus behave as a skin sensitizer, were tested on the patient. The patient reacted positively to the synthetic fragrance p ‐ t ‐butyl‐α‐methylhydrocinnamic aldehyde (Lilial ® ), a widely used fragrance compound not present in the fragrance mix. The combination of bioassay‐guided chemical fractionation and chemical analysis/structure‐activity relationships seems to be a valuable tool for the investigation of contact allergy to fragrance materials.